Keywords
N-acetyl-L-tryptophanamide (NATA)
tryptophan
fluorescence
phosphorescence lifetime
How to Cite
Abstract
This work is devoted to study how immobilization in the PVA films affects the fluorescence and phosphorescence lifetime of indole and its derivatives. The obtained results indicated that immobilization of the studied indoles in the PVA matrix, which leads to the increased microrigidity of the environment around the indole moiety, results in the increase of singlet and triplet excited state lifetime of the studied compounds. Most probably, the enhancement of the rigidity of the environment near the chromophore reduces the rates of the non-radiative deactivation pathways, which leads to the increase of excited state lifetimes of the studied compounds.
References
Lakowicz JR,Protein fluorescence. In: Principles of fluorescence spectroscopy. 2nded. Kluwer Academic/Plenum Publishers, New York, USA 1999, pp. 446-485.
Szabo AG,Rayner D.M,Fluorescence decay of tryptophanconformers in aqueous solution.J Am Chem Soc1980, 101:554-563.
Millar DP.Time-resolved fluorescence spectroscopy.Curr Opin Struct Biol 1996, 6:637-642.
Strambini EG, Strambini GB,Tryptophan phosphorescence as a monitor of protein conformations in molecular films.Biosens Bioelectron2000,15:483-490.
Strambini GB, Gonnelli M,Tryptophan phosphorescence in fluid solution.JAm Chem Soc 1995, 117:7646-7651.
Strambini GB., Kerwin BA, Mason BD.Gonelli M,The triplet-state lifetime of indole derivatives in aqueous solution.PhotochemPhotobiol 2004, 80:462-470.
Fischer CJ, Gafni A, Steel DG, Schauerte JA,The triplet-state lifetime of indole in aqueous and viscous environments: significance to the interpretation of room temperature phosphorescence in proteins.J Am Chem Soc 2002, 124:10359-10366.
Bent DV, Hayon E,Excited state chemistry of aromatic amino acids and related peptides. III. Tryptophan.J Am Chem Soc 1975, 97:2612-2619.
Tsentalowich Y, Snytnikova OA, Sagdeev RZ,Properties of excited states of aqueoustryptophan.J Photochem Photobiol A Chem 2004, 162:371-379.
Strambini GB, Gonnelli M.Tryptophan phosphorescence in fluid solutions. J Am Chem Soc 1995, 117:7646-7651.
Banks DD, Kerwin BA,A deoxygenation system for measuring protein phosphorescence.AnalBiochem2004, 324:106-114.
Strambini GB, Gonelli M,The indole nucleus triplet-state lifetime and its dependence on solvent microviscosity.Chem PhysLett1985,115:196-204.
Dashnau JL, Zelent B, Vanderkooi JM, Tryptophan interactions with glycerol/water and trehalose/sucrose cryosolvents: infrared and fluorescence spectroscopy and ab initiocalculations.BiophysChem 2005, 114:71-83.
Rung-Shu C, Yi-Jane C,Min-Huey C, Tai-Horng Y,The behavior of rat tooth germ cells on poly(vinyl alcohol).ActBiomater 2009, 5:1064-1074.
Kuijt J, Ariese F, Brinkman UAT, GooijerC,Room temperature phosphorescence in the liquid state as a tool in analytical chemistry.Anal Chim Acta 2003, 488:135-171.
Williams ATR., Winfield SA, Miller JN,Relative fluorescence quantum yields usinga computer controlled luminescence spectrometer.Analyst 1983, 108:1067-1068.
Somers KRF, Kryachko ES, Ceulemans A,Theoretical study of indole: protonation,indolyl radical, tautomers of indole, and its interaction with water.ChemPhys 2004, 301:61-79.
KamathSD, Kartha VB, MahatoKK,Dynamics of L-tryptophan in aqueous solution by simultaneous laser induced fluorescence (LIF) and photoacoustic spectroscopy (PAS). SpectrochimAct A 2008, 70:187-19.
Ryuzi K,Dependence of photoionization quantum yield of indole and tryptophan inwater on excitation wavelength.JPhotochemPhotobiolA2007, 189:211-217.
Feng S,Zong W, Liu R, Chai J, Liu Y,Micro-environmental influences on the fluorescence of tryptophan.Spectrochim ActA, 2010, 76:142-145.
Sau AK, Mitra S,Steady state and picosecond time-resolved fluorescence studies on native, desulpho and deflavo xanthine oxidase.BiochimBiophysAct 2000,1481:273-282.
Kirby EP, Steiner RF,The influence of solvent and temperature upon the fluorescence of indole derivatives. J Phys Chem 1970, 74:4480-4490.
Singh AK, Aruna RV,Fluorescence studies of tryptophan and tryptophan-retinol Shiff base in reverse micellar matrix. J Photochem Photobiol A1995, 89:247-250.
Qiang L, SeegerS,Label-free detection of protein interactions using deep UV fluorescence lifetime microscopy.AnalBiochem 2007, 367:104-110.
Zelent B, Kuśba J, Gryczynski I, Johnson MJ, LakowiczJR,Time-resolved and steady state fluorescence quenching of N-acetyl-L-tryptophanamide by acrylamide and iodide.Biophys Chem 1998, 73:53-75.