Methods of allergen detection based on DNA analysis
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Keywords

PCR allergens
hazelnut
peanut
charlock
celery
sesame lupine
walnut
almond
macadamia nut
hickory
pistachio
wheat

How to Cite

Słowianek, M., & Majak, I. (2011). Methods of allergen detection based on DNA analysis. Biotechnology and Food Science, 76(2), 39-44. https://doi.org/10.34658/bfs.2011.75.2.39-44

Abstract

Many allergens, such as hazelnut, peanut, charlock, celery, sesame, lupine, walnut, almond, macadamia nut, hickory, pistachio, wheat gliadins, may be present in food products, however, undeclared or as unintentional additives. Due to the growing number of allergic reactions, it is crucial to have fast, reliable methods of allergen detection in processed food products. This review summarizes the recent methods of allergen detection in food products based on PCR reactions, namely PCR-ELISA, Real-time PCR, PCR-PNA-HPLC, Duplex PCR and Multiplex Real-time PCR, describing their principles, applications, detection limits, drawbacks and advantages.

https://doi.org/10.34658/bfs.2011.75.2.39-44
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