Keywords
laying hens
antibiotic resistance
rearing system
How to Cite
Abstract
Salmonelloses are among the most common animal-borne infections. The most frequent causes of their occurrence are contaminated chicken eggs. For this reason, the control of bacteria from the Salmonella genus living in the cloaca of laying hens can contribute to the reduction of spreading levels of infections with these bacteria. The most popular methods of rearing laying hens in small agricultural farms comprise run and cage keeping. The aim of the performed studies was to determine the impact of rearing and nutritional systems on the occurrence of egg infections with Salmonella spp. bacteria. Detection by PCR method corroborated presence of bacteria from the Salmonella genus on eggs surface from hens kept in litter system. Latex serotyping test confirmed the presence of Salmonella Enteritidis. Salmonella spp. was not detected on eggs surface from hens kept in cage system. Salmonella spp. isolates from the eggs surface of hens reared on litter were characterized by drug resistance to tetracycline. Addition of EM probiotic failed to show reduction in incidence of Salmonella spp. infection.
References
Crespo PS, Hernandeze G, Echeita A, Torres A, Ordenez P, Aladuena A. Surveillance of foodbourne disease outbreaks associated with consumption of eggs and egg products: Spain. 2002-2003, Eurosurveillance Weekly 2005, 10: 24.
De Jong B, Ekdahl K. Human salmonellosis in travelers is highly correlated to the prevalence of Salmonella in laying hen flocks. Eurosurveillance Weekly 2006, 11: 7.
Van Hoorebeke S, Van Immerseel F, De Vylder J, Ducatelle R, Haesebrouck F, Pasmans F, Kruif A, Dewulf J. The age of production system and previous Salmonella infections on-farm are risk factors for low-level Salmonella infections in laying hen flocks. Poult Sci 2010, 89: 1315-1319.
García C, Soriano J, Benitez V, Catalá – Gregor P. Assessment of Salmonella spp. in feces, cloacal swabs, and eggs (eggshell and content separately) from a laying hen farm. Poult Sci 2011, 7: 1581-1585.
The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2010., 2012. EFSA Journal, 10, 2597, p. 442.
CLSI Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. CLSI 950 West Valley Rad, Suite 2500,Wayne Pennsylvania. 2013, 33 (1): 47-49.
Tellez G, Pixley C, Wolfenden RE, Layton SL, Hargis BM. Probiotics/direct fed microbials for Salmonella control in poultry. Food Research Internat 2012, 45: 628-633.
Van Hoorebeke S, Van Immerseel F, Haesebrouck F, Ducatelle R, Dewulf J. The Influence of the Housing System on Salmonella Infections in Laying Hens: a review. Zoonoses and Public Health 2011, 58: 304-311.
Council directive 1999/74/EC of 19 July 1999 laying down minimum standards for the protection of laying hens.
Bouzidi N, Aoun L, Zeghdoudi M, Bensouilah M, Elgroud R, Oucief I, Granier SA, Brisabois A, Desquilbet L, Millemann Y. Salmonella contamination of laying-hen flocks in two regions of Algeria. Food Research Int 2012, 45: 897-904.
Gast RK, Guraya R. Jones DR, Anderson KE. Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages. Poult Sci 2013, 92: 468-473.